Journal of Chromatography & Separation Techniques
Open Access

Abstract

Purification of Avian IgY with Trichloroacetic Acid (TCA)

Hellen Asemota, Suzette Curtello, Angel Alberto Justiz Vaillant, Wayne Mohammed, Sehlule Vuma, Chalapathi Rao AV, Arvind Kurhade, Shivnarine Kissoon, Monica Smikle, Brian Wisdom and Geeta Kurhade

By using trichloroacetic acid (TCA) precipitation to separate egg yolk proteins, mainly IgY was demonstrated that this procedure yield twice more IgY that its counterpart polyethylene glycol (PEG). An ELISA demonstrated that it does not affect the functionality of the IgY molecule. The TCA precipitation procedure provides an efficient and rapid way to purify IgY antibody. It can be used for large scale production of high quality IgY.