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Micellar High Performance Liquid Chromatographic Method for Simultaneous Determination of Clonazepam and Paroxetine HCl in Pharmaceutical Preparations Using Monolithic Column | Abstract
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+32 25889658

Abstract

Micellar High Performance Liquid Chromatographic Method for Simultaneous Determination of Clonazepam and Paroxetine HCl in Pharmaceutical Preparations Using Monolithic Column

Fawzia Ibrahim, Nahed El-Enany, Shereen Shalan and Rasha Elsharawy

A new and accurate micellar high performance liquid chromatographic method coupled with ultraviolet detection was developed for simultaneous determination of Clonazepam (CLZ) and Paroxetine (PRX) using a monolithic C-18 column, and mobile phase consisting of 0.175M Sodium dodecyl sulphate, 12% n-propanol prepared in 0.02M phosphoric acid at pH 6.0. The analysis was performed at a flow rate of 1 mL/min with ultraviolet- detection at 300 nm. The method was linear over the concentration range (1.0-20 μg) and (4.0-250 μg) with limits of detection of 0.277, 2.675 μg/mL and limits of quantification of 0.838, 8.106 μg/mL for CLZ and PRX respectively. The average % recovery was found to be 100.08 ± 1.31 and 100.22 ± 1.15 for CLZ and PRX respectively. Method validation according to ICH Guidelines recommendation was evaluated. Statistical analysis of the results obtained by the proposed method was compared successfully with those obtained using the reference one. There was no significance difference between the two methods regarding accuracy and precision respectively.

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