Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064


Method Development and Validation of Tablet Dosage form Containing Losartan, Atenolol and Hydrochlorthiazide Using Internal Standard by RPHPLC

Tengli AR and Gurupadayya BM

A simple, sensitive and specific RP-HPLC method with UV detection was developed for the simultaneousestimation of losartan, atenelol and hydrochlorothiazide in tablet dosage form and telmisartan as an internal standard. Separation was achieved with an phenomenex luna 5 μ CN 100R, 250×4.60 mm 5 micron size column, ambient temperature with a low pressure gradient mode with mobile phase containing acetonitril, and 0.2% of diammonium hydrogen orthophosphate buffer pH 2.8 adjusted with orthophosphoric acid (50:50). The flow rate was 1 mL min-1 and eluent was monitored at 230 nm. The selected chromatographic conditions were found to effectively separate losartan (LOSAT), atenelol (ATEN) and hydrochlorothiazide (HCT) with retention time of 6.0, 5.0 and 4.0 min respectively. The proposed method was found to be rectilinear over the range of 25-150 μgmL-1, 25-150 μgmL-1 and 6.25-37.50 μgmL-1 for losartan, atenelol and hydrochlorothiazide respectively. The proposed method was found to be accurate, precise, reproducible and specific and it can also be used for routine quality control analysis of these drugs in biological samples either alone or in combined pharmaceutical dosage forms