Journal of Chromatography & Separation Techniques
Open Access

Abstract

Identification and HPLC purification of Aspirin (Acetylsalicylic acid) from the seed coats, leaves and bark of Givotia Rottleriformis Griff.

Samuel Kamatham*

Aspirin (Acetylsalicylic acid; ASA) an anti-inflammatory and anti-cancer molecule derived from Salicylic Acid (SA) use to relieves pain, swelling, cold or flu, prevent colorectal cancer and cardiovascular diseases. ASA induces defense response against wide range of pathogens in plants. Recently, we reported Benzoyl Salicylic Acid (BzSA) and other anti-cancer molecules such as Gallic Acid (GA) methyl gallate from the seed coats of G. rottleriformis. The present study reporting the detection of natural aspirin for the first time in the seed coats, leaves and bark of G. rottleriformis. HPLC Separation chromatogram of hexane extract of seed coats, leaves and bark shown a peak at RT 24.8 min coeluted with the aspirin standard. The purified aspirin from the seed coats were subjected to 1H NMR and were confirmed as aspirin. These results suggest that aspirin biosynthesis is taking place in seed coats, leaves and bark of G. rottleriformis and supports the medicinal properties of G. rottleriformis. These results suggest that the seed coats, leaves and bark of this plant contains the highly useful medicinal compounds to treat the rheumatism, cancer, cardiovascular, psoriasis, anti-inflammatory and other skin diseases.

Published Date: 2022-09-20; Received Date: 2022-08-19