Journal of Chromatography & Separation Techniques
Open Access

Abstract

HPLC-UV Analysis of Phloretin in Biological Fluids and Application to Pre-Clinical Pharmacokinetic Studies

Connie M. Remsberg, Jaime A. Yáñez, Karina R. Vega-Villa, Nicole D. Miranda, Preston K. Andrews and Neal M. Davies

A method of analysis of phloretin [3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one] in biological fl uids is necessary to study the kinetics of in vitro and in vivo metabolism and its concentration in natural products. A high- performance liquid chromatographic (HPLC) method was developed for the determination of phloretin in rat serum. Separation was achieved on a Chiralcel ® OD-RH column with UV detection at 288 nm. The calibration curves were linear ranging from 0.5 to 100  g/ml. The mean extraction ef fi ciency was >95%. Precision of the assay was <14%, and was within 10.9% at the limit of quantitation (0.5  g/ml). Bias of the assay was lower than 14%, and was within 9.22% at the limit of quantitation. The HPLC method was successfully applied to the pharmacokinetic study of phloretin in rats.