Journal of Chromatography & Separation Techniques
Open Access

Abstract

Detection of Phytoconstituents of Medicinal Plant Terminalia arjuna Using Chromatographic Techniques

Chitte RR, Nagare SL, Date PK and Shinde BP

The plant extract revealed the presence of phytochemicals such as Phlobatannins, phenols, leucoanthocyanins, saponins, emodins, coumarins and quinones. Process of extraction of pure compound using column chromatography. The gradient of solvent eluted fraction has in pure form, tested and partial characterized. Thin layer chromatographic study was carried out by using various solvent system of varying polarity of which water, ethyl acetate and propanol system suited the best. In vitro anti-inflammatory activity was evaluated using albumin denaturation (50%) membrane stabilization assay (75%) and proteinase inhibitory activity (33.33%). For anti-inflammatory activity Aspirin (85.67%) used as standard drug. Using alfa amylase inhibition assay, In vitro antidiabetic activity was determined, fraction 5 (89.12%) and fraction 6 (80%) were showed at conc. 500 μg/mL. Antimicrobial efficiency of the plant extract fractions was determined using well diffusion method against Pseudomonas sp., Bacillus sp., Protease and Staphylococcus aureus, of which no activity was observed against Pseudomonas sp.