Applied Microbiology: Open Access
Open Access
ISSN: 2471-9315
+44 1300 500008
ISSN: 2471-9315
+44 1300 500008
Elisa Bona
Universita del Piemonte Orientale, Italy
Keynote: Appli Microbiol Open Access
Vitis vinifera is a Mediterranean crop with relevant impact on the Italian landscape, culture and economy. The rhizosphere is a
hotspot where the release of root exudates modulates bacterial density and diversity. Despite the high bacterial number found in
rhizosphere, only 1-10% of the soil bacteria are able to grow on laboratory media, so that the majority of the bacterial cells in soil results
to be viable, unculturable, but active; even more, this percentage change according to the host plant. Thanks to culture-independent
methods (metagenomics) the complexity of the soil/rhizosphere microbial community has been explored. However, metagenomics
doesn��?t provide information regarding the activity and the molecular interactions between the bacterial communities and roots. Proteins
are the drivers of cellular activities encoded by the genome. Therefore, proteomic tools could be useful to gain information about microbial
community activity and to better understand the real interactions pathways between roots and soil. A comparison between the microbial
community structure in rhizosphere and bulk soil using metagenomics (pyrosequencing of 16S rDNA) and proteomics (MS/MS analysis
of the total protein occurring in soil samples) was performed. Actinobacteria were the dominant class in all the soil samples, followed by
Proteobacteria, Gemmatimonadetes and Bacteroidetes. While Actinobacteria and Proteobacteria are well known as dominant in soil, for the
first time, members belonging to Gemmatimonadetes have been observed in vineyard soils. Bacteria belonging to Streptomyces, Bacillus,
Bradyrhizobium, Burkholderia and Pseudomonas genera were the most active. Concerning the biological activity of these genera, in the
rhizosphere (Figure 1), we observed the exclusive presence of phosphorus metabolic process and the regulation of: biosynthetic, cellular,
macromolecule, nitrogen compound and primary metabolic process. Our results underlined the difference between the metagenomic and
metaproteomic approaches and the potentiality of proteomics in describing the environmental bacterial communities and their activity.
Recent Publications
1. Bona E., Todeschini V., Cantamessa S., Cesaro P., Copetta A., Lingua G., Gamalero E., Berta G., Massa N (2018). Combined
bacterial and mycorrhizal inocula improve tomato quality at reduced fertilization. Scientia Horticulturae 234, 160��?165.
2. Novello G., Gamalero E., Bona E., Boatti L., Mignone F., Massa N., Cesaro P., Lingua G., Berta G (2017). The rhizosphere bacterial
microbiota of Vitis vinifera cv. Pinot Noir in an integrated pest management vineyard. Frontiers in Microbiology, 8, 1528.
3. Gamalero E., Marzach�?¬ C., Galetto L., Veratti F., Massa N., Bona E., Novello G., Glick B. R., Ali S., Cantamessa S., Dâ�?�?Agostino G.,
Berta G (2017). An 1-aminocyclopropane-1-carboxylate (ACC) deaminase-expressing endophyte increases plant resistance to
Flavescence Dor�?©e phytoplasma infection. Plant Biosystems, 151 (2), 331-340.
4. Bona E., Cantamessa S., Massa N., Manassero P., Marsano F., Copetta A., Lingua G., D��?Agostino G., Gamalero E., Berta G (2017).
Arbuscular mycorrhizal fungi and plant growth-promoting pseudomonads improve yield, quality and nutritional value of
tomato: a field study. Mycorrhiza, 27, 1-11.
Elisa Bona. Researcher in Microbiology at the Università del Piemonte Orientale. She holds a Specialization degree in Microbiology and Virology (2010) at the Università di Genoa and a Ph.D. (2006) in Environmental Science at the Università del Piemonte Orientale. She is teacher in different courses at UPO University: since 2015, Professor of General Microbiology, Professor of Applied Bacteriology and Professor Diagnostic in Microbiology. (AA 2013-2014) Professor of Environmental Microbiology and Professor of Agri-Environmental Microbiology.
E-mail: elisa.bona@uniupo.it