GET THE APP

Unusual transglucosylation reaction on a soybean isoflavone by amylosucrase from two different bacterial sources
Applied Microbiology: Open Access

Applied Microbiology: Open Access
Open Access

ISSN: 2471-9315

Unusual transglucosylation reaction on a soybean isoflavone by amylosucrase from two different bacterial sources


12th International Congress on Microbial Interaction and Applications of Beneficial Microbes

July 17-18, 2017 Munich, Germany

Eun-Ryoung Kim, Jung-Min Choi, Chan-Su Rha, Young-Sung Jung, Gi-Tae Kim, Min-Ji Ko, Dae-Ok Kim and Cheon-Seok Park

Kyung Hee University, Republic of Korea

Posters & Accepted Abstracts: Appli Micro Open Access

Abstract :

Amylosucrase (ASase, EC 2.4.1.4) belongs to glycoside hydrolase family 13 (GH13) and has uncommon three enzymatic activities of hydrolysis, polymerization and isomerization. ASase acts on substrates containing ?±-glucoside linkages and has a catalytic mechanism named ?±-retaining double displacement forming glycosyl-enzyme intermediate. In this study, two kinds of recombinant ASases from Neisseria polysaccharea (NPAS) and Deinococcus geothermalis (DGAS) were used for transglucosylation on daidzin. Daidzin is known as a major isoflavone and found in Kudzu and soybean. Transglucosylation of daidzin is expected to improve watersoluble, stability of chemical structure and taste. Two similar enzymes (NPAS and DGAS) showed quite different reaction products with same substrates. While the main transglycosylation products of NPAS reaction were daidzein-7-O-?²-diglucoside and daidzein- 7-O-?²-triglucoside, those obtained by DGAS were daidzein-7-O-?²-diglucoside and daidzin isomer. These results indicated that two enzymes may have different structural spaces inside the catalytic sites, which lead to the uncommon transglycosylation reactions.

Biography :

Eun-Ryoung Kim graduated from Chung Buk National University and now has been in the Master’s course in Food Microbiology and Biotechnology at Kyung Hee University.

Email: [email protected]

Top