Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Virginia Sede�o-Monge, Ra�l Arcega-Revilla, Emmanuel Rojas Morales, Gerardo Santos-L�pez, Juan Carlos Perez-Garc�a, Francisca Sosa Jurado, Ver�nica Vallejo-Ruiz, Casandra Lucrecia Solis Morales, Salvador Aguilar-Rosas and Julio R Reyes-Leyva
Posters: J Clin Cell Immunol
Multiple sclerosis (MS) is an autoimmune disease characterized by a triad of inflammation, demyelination and gliosis. Some experimental observations suggest that IFN- γ and other proinflammatory cytokines have an important role in the pathogenesis of MS. The suppressors of cytokine signaling (SOCS) negatively regulate the proinflammatory cytokine- induced response, moderating consequently the immune response. The aim of the study was to quantify SOCS1 and SOCS3 transcription in peripheral blood leucocytes of patients with MS by quantitative real-time reverse transcription-PCR and analyze their correlation with disease progression. Values were analyzed by means of the comparative method 2-ΔΔCT (fold difference). Forty MS patients and twenty six healthy individuals were included; all persons were neurologically evaluated through the Expanded Disability Status Scale (EDSS). SOCS1 transcription decreased significantly in MS patients compared with neurologically healthy persons (0.08�0.02 vs. 1.02�0.23; p=0.0001); while SOCS3 transcription increased in MS patients compared with controls (2.76�0.66 vs. 1.03�0.27; p=0.0008). A weak correlation between the patients neurological status (EDSS values) and either SOCS1 or SOCS3 expression was found (for SOCS1 r=-0.11 p= 0.5; for SOCS3 r= -0.2 p=0.2) and slight negative correlation between SOCS3 and SOCS1 expression was found (Spearman?s r=-0.57; p=0.0003) in MS patients. An important contribution of our study is that the ratio SOCS1:SOCS3 showed a strong and significant association between both genes in all samples, this can be a reflection of the fine balance between different members of SOCS family.
Virginia Sede�o Monge completed his PhD in Ciencias Microbiol�gicas in 2010 from Universidad Aut�noma de Puebla. She is Professor of Physiology in the Universidad Popular Aut�noma del Estado de Puebla School of Medicine. She is member of Sistema Nacional de investigadores (SNI) of M�xico since 2012, and is currently working on transcription genes with real time PCR.