TLR2 located in macrophage sub cellular compartments negatively r | 53304
Journal of Clinical and Cellular Immunology

Journal of Clinical and Cellular Immunology
Open Access

ISSN: 2155-9899

+44 1223 790975

TLR2 located in macrophage sub cellular compartments negatively regulates TLR4 mediated inflammatory response to internalized bacteria

Conference Series LLC Joint International Event on 5th European Immunology & Innate Immunity

July 21-23, 2016 Berlin, Germany

Ludovica F Butto, Silvia Melgar, John OCallaghan, Ian B Jeffery, Hugh M B Harris, John MacSharry, Fergus Shanahan, Paul W OToole and Ken Nally

Technische Universit�?�?�?¤t Munchen Weihenstephan, Germany
National University of Ireland, Ireland

Posters & Accepted Abstracts: J Clin Cell Immunol

Abstract :

Recognition of microbes by toll-like receptors (TLRs) is critical for initiation of appropriate innate and adaptive immune responses. How crosstalk between TLRs situated in various cellular locations contributes to host-microbe dialogue and immunoregulation is presently unclear. Here we report a dual role for TLR2 in regulating the response of macrophages to internalized bacteria. Cell surface TLR2 initiated an inflammatory response while sub cellular TLR2 negatively regulated a TLR4-mediated hyper-inflammatory response that was phagocytosis dependent and driven by a type-I interferon autocrine loop. TLR2 deficient macrophages were hyper responsive to commensal and pathogenic bacteria and developed an M1 like phenotype, which was absent in TLR2/TLR4 deficient macrophages. Our findings identify sub cellular TLR2 as being important for the negative regulation of inappropriate TLR4 mediated inflammatory responses to bacteria.