Kuanhou Mou, Pan Li and Wei Liu
The First Affiliated Hospital of Xi’an Jiaotong University, China
Posters & Accepted Abstracts: J Clin Exp Dermatol Res
Vitiligo, a common idiopathic acquired depigmentation disorder, occurs mostly in young people who are typically very concerned about their appearance. Although the causes of vitiligo are still unknown, so, it is still not easy to treat with. In the past, scholar always focused on abnormal of melanocytes (MC), but recently we found not only MC but also keratinocytes had malfunctions. For example, HMGB1 could be passively released by apoptosis and necrosis keratinocytes or actively released in vitiligo. In order to investigate the release of HMGB1 by HaCaT cell and keratinocytes of tissue from vitiligo patients, HaCaT cells were treated with UV light or with apoptosis drugs or with cytokines and freeze-thaw process. The supernatant was condensed and subjected to SDSPAGE to detect the release of HMGB1. Measure the HMGB1and cleaved caspase-3 expression in skin biopsies of normal control subjects and vitiligo patients by immunofluorescence. HMGB1 was detected in the supernatant of HaCaT after treated and control supernatant was not detected. Cleaved caspase-3 was found in the stratum corneum of vitiligo patients and control patient was not found. HMGB1 was expressed in the nucleus of keratinocytes of control patients and transferred from nucleus to cytoplasm in vitiligo patients.
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