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The relic legume Oxytropis popoviana forms symbiosis with rhizobi | 38312

Applied Microbiology: Open Access
Open Access

ISSN: 2471-9315

+44 1300 500008

The relic legume Oxytropis popoviana forms symbiosis with rhizobial co-microsymbionts having complement sets of symbiotic genes


12th International Congress on Microbial Interaction and Applications of Beneficial Microbes

July 17-18, 2017 Munich, Germany

Safronova Vera

ARRIAM, Russia

Posters & Accepted Abstracts: Appli Micro Open Access

Abstract :

The legume Oxytropis popoviana Peschkova belongs to the Miocene-Pliocene relic and grows in restricted habitats of the Baikal Lake region. Microsymbionts of this rare and endemic plant species have never been isolated, although the study of relict symbiotic systems is very important in the context of evolution of plant-rhizobia interactions. The aim of this work was to obtain root nodules of O. popoviana under laboratory conditions, to get and identify rhizobial isolates as well as to search their symbiotic genes. Pot experiment was conducted using soil and seeds collected in distribution area of this legume. Plants were cultivated for 60 days in the growth chamber and nodules were collected. Identification of bacterial isolates was performed by the 16S rRNA gene sequencing method. Genomes of two strains were sequenced by the Illumina MiSeq genome sequencer. Symbiotic genes were searched with CLC Genomics Workbench 7.5.1. Total of 18 strains belonging to 4 rhizobial genera (Mesorhizobium, Bradyrhizobium, Rhizobium and Bosea) were isolated from nodules obtained in the pot experiment. Some nodules contained a pair of different strains. Two strains isolated from the same nodule were most closely related to the species Mesorhizobium erdmanii and Bradyrhizobium betae. Analysis of whole genome sequences of these strains showed that they had different sets of symbiotic genes. Thus we hypothesize that the pair of strains isolated from root nodule of O. popoviana can be ancestral rhizobial co-microsymbionts and their simultaneous presence in roots is required for efficient nodulation of this relic legume.

Biography :

Email: v.safronova@rambler.ru

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