Applied Microbiology: Open Access
Open Access
ISSN: 2471-9315
+44 1300 500008
ISSN: 2471-9315
+44 1300 500008
Noushin Marhamati
Shahid Beheshti University of Medical Sciences, Iran
Posters & Accepted Abstracts: Appli Micro Open Access
Background & Aim: Methicillin Resistance Staphylococcus aureus (MRSA) is a type of Staphylococci that is resistant to the antibiotics such as methicillin, cloxacillin, dicloxacillin, naficillin and cephalosporins. The hlg is an important gene in MRSA strains of S. aureus. Gamma-hemolysin is toxic for human erythrocytes. In present study, we sought to examine the prevalence of MRSA strains of S. aureus and detect the gene of hlg in health care staff. Materials & Methods: The descriptive study was conducted from January to December 2014. In this survey, 229 nose specimens were taken from the health care staff of Mofid Children Hospital. The isolates were identified as S. aureus based on biochemical and phenotypical tests. To determine the profile of antibiotic resistance of S. aureus isolates, the disk diffusion method (Kirby-Bauer) was used according to 2013 CLSI guidelines. The PCR assays were used for detection of hlg. Results: From 229 health care staff, 200 (87.33%) were female and 29 (12.66%) were male. Out of 229 samples, 27 (12%) isolates were positive for S. aureus of which 21 (77.7%) were MRSA and 6 (22.3%) were MSSA (Methicillin Sensitive S. aureus). PCR assays for detection of hlg were used. Overall, 18 (85.71%) of MRSA isolates were positive for the presence of hlg. Conclusion: In conclusion, gamma-hemolysin appears to be a more possible virulence factor than other virulence factors in MRSA isolates.
Email: fafallah@sbmu.ac.ir