Journal of Clinical and Cellular Immunology

Journal of Clinical and Cellular Immunology
Open Access

ISSN: 2155-9899

+1 504 6082390

The in vitro effect of chondroitin sulfate and glucosamine on tissue-engineered nucleus pulposus

International Conference on Clinical & Cellular Immunology

October 22-24, 2012 DoubleTree by Hilton Chicago-Northshore, USA

Miroslav Malkovsky

Accepted Abstracts: J Clin Cell Immunol

Abstract :

Most vaccine strategies are designed to elicit adaptive immune responses to a variety of microbial or tumor-associated antigens and target predominantly α β T cells and B cells. In contrast to these cells, Vγ9Vδ2 T lymphocytes typically recognize nonpeptidic antigens generated by the DOXP (many eubacteria, algae, plants, apicomplexa) and mevalonate (eukaryotes, archaebacteria and certain eubacteria) pathways of isoprenoid synthesis. Vγ9Vδ2 T cells can be also activated by certain nitrogen-containing bisphosphonates (N-BPs). These activated Vg9Vd2 T cells can kill very effectively various tumor and virus-infected cells. We have shown that intravenous administration of N-BPs combined with low doses of IL-2 induces a large pool of CD27+ and CD27-effector/memory Vγ9Vδ2 T cells in the peripheral blood. In AIDS patients, these events are associated with decreases of the peripheral blood virus load. However, Vg9Vd2 T lymphocytes exposed to IL-2, IL-15 and TGF-b display regulatory functions in vitro typically associated with ab CD4+CD25+ Tregs. Vg9Vd2 Tregs derived by this method express the transcription factor FOXP3 and, similar to ab Tregs, suppress the proliferation of anti-CD3 and anti-CD28 stimulated-PBMCs in the presence of IL-2. The presence of high numbers of these cells may interfere with anti-cancer or anti-infectious immune responses. Several clinical trials focused on γδ T-cell activation in vivo in patients with various cancers are currently in progress. The findings from these trials could guide novel combinations of suitable Vg9Vd2 T-cell activations with conventional therapies that may further improve the armament of clinical oncologists as well as specialists in infectious diseases.

Biography :

Miroslav Malkovsky received his Ph.D in 1979 from Charles University, Prague. At present he is Professor for Department of Medical Microbiology and Immunology, University of Wisconsin Medical School; University of Wisconsin Comprehensive Cancer Center; and Wisconsin Regional Primate Research Center (WRPRC), Madison, Wisconsin.