Journal of Drug Metabolism & Toxicology
Open Access
ISSN: 2157-7609
ISSN: 2157-7609
Sedigheh Rafiei Tabatabaei
Shaheed Beheshti University, Iran
Scientific Tracks Abstracts: J Drug Metab Toxicol
Background: Serious infections by Pseudomonas aeruginosa are commonly treated with the combination of a beta-lactam antibacterial and an aminoglycoside. Therefore, production of a 16S rRNAmethylase may result in an extremely important antibacterial resistance profile. Objectives: The present study was conducted to determine the prevalence of Cephamycinase (CMY), aminoglycoside 6â�?�?-N-acetyltransferase (aac(6â�?�?)-Ib) and 16S rRNAmethylase genes among Pseudomonas aeruginosa isolates from Iran. Patients & Materials: This descriptive study was performed on hospitalized burnt patients during 2011 and 2012. Antibiotics susceptibility tests were performed by disc diffusion and broth microdilution methods. CMY, aac(6â�?�?)-Ib, 16S rRNAmethylase genes were detected by PCR method. Results: Seventy-seven (77%) of 100 isolates were resistant to Imipenem and Ceftazidime. aac(6)-Ib, Cephamycinase (CMY), and rRNAmethyltransferase (rmtB and rmtD) were detected in 57 (74.02%), 7 (9.09%), 11 (14.28%), and 9 (11.68%) isolates, respectively. PCR results for aminoglycoside resistance methyltransferase (armA) and rRNAmethyltransferase (rmtC) were all negative. Aminoglycoside resistance methyltransferase (armA), and rRNAmethyltransferase (rmtC) were not detected. Conclusions: This study detected multiple drug resistance in Pseudomonas aeruginosa including resistance to �?²-lactams, Aminoglycosides, and Fluoroquinolones. Therefore, identification of drug resistance patterns in P. aeruginosa and detection of pan-resistant producing isolates are of great importance in prevention and control of infections in burn center ward.
Email: srt.tabatabaei@gmail.com