Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
ISSN: 2167-0412
Zayan Nabilah Rasyidah bt Abd Razak
Universiti Teknologi MARA, Malaysia
Posters & Accepted Abstracts: Med Aromat Plants
A. paniculata leaves are known to have antimicrobial activities and it is widely used as traditional medicine worldwide. This study investigated the antimicrobial activities of methanolic extract of S. aureus (ATCC 25923) by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), time-kill assay and the effects of A. paniculata extract on S. aureus in SDS-PAGE analysis. Several concentration of the A. paniculata extracts were used to determine MIC value and the lowest concentration that can inhibit growth of S. aureus were further tested to obtain MBC value. Time-kill assay was performed in 24 hours to determine the incubation period needed for A. paniculata extract to completely kill S. aureus. The treated samples (A. paniculata extract and S. aureus) and untreated samples (S. aureus only) were compared in terms of appearance of cytoplasmic protein bands to determine the effect of treatment. A. paniculata extract showed high antimicrobial activity against S. aureus with MIC and MBC value was 0.06 mg/ml. The MIC value was able to completely killed S. aureus in 24 hours incubation time. Lipase (76 kDa) was identified on SDS-PAGE analysis which was inhibited when treated with A. paniculata extract. The observation suggests that a possible mechanism by which methanolic extract of A. paniculata inhibits S. aureus by lowering production of an important protein, lipase.
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