Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
ISSN: 2167-0412
Haw-Wen Chen and Hui-Chun Chen
China Medical University, Taiwan
Posters & Accepted Abstracts: Med Aromat Plants
Pathogenesis of insulin resistance has been linked to obesity-induced chronic low grade inflammation. In this study, we investigated the effects of andrographolide (AND), 14-deoxy-11,12-didehydro-andrographolide (deAND), and ethanolic extract of Andrographispaniculata (APE) on improvement of insulin sensitivity induced by TNF-�?±in mature 3T3-L1 adipocytes and in high-fat diet (HFD)-fed C57BL/6J miceand characterize theirworking mechanisms. In mature 3T3-L1 adipocytes, we found that APE and deAND reversed the TNF-�?±-induced suppression of insulin-stimulated 2-NBDG uptake. APE and deANDincreased protein level of glucose transporter 4 (GLUT4) in cell membrane fraction by phosphorylating IRS-1 tyr632 and AKT ser473 in TNF-�?±-treated 3T3-L1 adipocytes. In addition, AND, deAND, and APE inhibited TNF-�?±-induced inflammatory response by blocking p38 phosphorylation and NF-�?ºB p65 nuclear translocation. In vivo, we examinedchow diet or HFD with or without 0.1% AND, 0.05% deAND, or0.4% APE intervention for 16 weeks on insulin resistance.Dietary deAND and APE reduced HFD-induced body weight gain and improvedglucose intolerance and insulin sensitivity.AND, deAND and APE attenuated the levels of MCP-1 and GPT in the serum of HFD-fed mice.Meanwhile, dietary AND, deAND, and APE enhanced theexpression of GLUT4 in cell membrane fraction via phosphorylation of IRS-1 tyr632 and AKT ser473 in epididymal adipose tissue ofHFD-fed mice. These results suggest that A.paniculata possesses potential to fight against obesity-induced insulin resistance and inflammation.
Haw-Wen Chen has completed her PhD from the Department of Food Science and Human Nutrition of Iowa State University, USA. She is currently a Professor of the Department of Nutrition of China Medical University in Taiwan. She has published more than 80 papers in reputed journals.