Staphylococcal toxins (entrotoxins A,B,C,TSST-1) in synovial flui | 23436
Orthopedic & Muscular System: Current Research

Orthopedic & Muscular System: Current Research
Open Access

ISSN: 2161-0533


Staphylococcal toxins (entrotoxins A,B,C,TSST-1) in synovial fluid of patients with arthritis

2nd International Conference and Exhibition on Orthopedics & Rheumatology

August 19-21, 2013 Embassy Suites Las Vegas, NV, USA

Samileh Noorbakhsha

Posters: Orthop Muscul Syst

Abstract :

Back ground &Objective: Determination the etiologic causes in septic arthritis is very important. Goal of study was searching the staphylococcal entrotoxinsA, B, C and Toxic Shock Staphylococcal toxin-1 in synovial fluid of patients with arthritis Methods & materials: A cross sectional study done in pediatric and orthopedic ward of Rasoul hospital in Tehran; Iran (2008-2010). Gram stain; conventional culture; direct detection of soluble bacterial antigens (H.influ; S. pneumonia, group B streptococci, and N.meningitidis) by Latex particle agglutination tests;Staphylococcal supper antigens ( Enzymeimmune assay) upon synovial fluid of 62 cases (5 months-16 years; mean =11 � 3.8 years). P value < 0.05 considered statistically significant. Positive culture (n=11) included: S.Aureus 4; S.pneumonia 5; H.influ 1, Klebsiella 1 .10% of cases had positive gram stain;4 cases had positive Latex particle agglutination tests. Staphylococcal arthritis diagnosed in 7 (39%) of cases upon positive culture or positive gram stain in synovial fluid .The most common type of supper antigens was TSST-1(47%) ;entrotoxinsB(18%) was the less common type. Isolation of S.Aureus(positive culture) was related to presence of entrotoxinsA in synovial fluid but not for entrotoxins B, C and TSST-1. Conclusion: S.Aureus has a prominent role in arthritis. 47% of cases with negative culture for S.aureus had at least 1 type of Staphylococcal supper antigens in synovial fluid. Failure of isolation S.aureus and other organismsmight due to natural ungrowth of organism; previous antibiotic usage or low technical methods for isolation .Use of methods for searching the antigens of usual organisms, or staphylococcal supper antigens could helpful for treatment . Key Words: Arthritis; Septic Arthritis;Staphylococcalsupper antigens; Staphylococcal Entrotoxins (A, B, C), TSST-1 (Toxic Shock Staphylococcal toxin).