Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
ISSN: 2155-9554
Dilip Kachhawa
Dr. S.N. Medical College, India
Posters & Accepted Abstracts: J Clin Exp Dermatol Res
Background & Aim: Stable vitiligo can be treated by various surgical procedures. Non-cultured melanocyte grafting techniques were developed to overcome time consuming process of culture while at the same time providing acceptable results. All the techniques using non-cultured melanocyte transfer involve trypsinization as an integral step. Jodhpur technique used by the author is autologus, non-cultured, non-trypsinized, melanocyte and keratinocyte grafting. This is the only technique that does not require split thickness graft. Methods: At the donor site, dermabrasion was done with the help of micromotor dermabrader at slow rpm till pin point bleeding was seen. Dermabrasion was then continued till the upper dermis and then stopped. The paste like material obtained by this procedure containing melanocytes and keratinocytes was harvested with spatula and was subsequently spread over the recipient area. Recipient site was prepared in the same manner as donor site except no harvesting of epidermal particles was done. After 10 days dressing at both sites was removed taking utmost care at the recipient site as there was theoretical risk of dislodging epidermal cells. Results: In a study of 437 vitiligo patches, more than 75% re-pigmentation (excellent improvement) was seen in 41% of the patches. Lesions on thigh (100%), face (75%) and trunk (50%) showed maximal excellent improvement while patches on joints and acral areas did not show much improvement. Conclusions: This technique is a cheaper, less time consuming alternative to other techniques which involve tryspsinization of melanocytes and at the same time provides satisfactory uniform pigmentation.
Email: drdilipkachhawa@hotmail.com