Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Daisy Vanrompay, Evelien De Clercq, Matthias Van Gils, Katelijn Schautteet, Bert Devriendt, Koen Chiers, Eric Cox and Deborah Dean
Ghent University, Belgium
Children��?s Hospital Oakland, USA
Posters & Accepted Abstracts: J Clin Cell Immunol
We evaluated the pathogenesis, pathology and immune response of a female genital tract infection with Chlamydia trachomatis L2c, the most recently discovered lymphogranuloma venereum strain, using a porcine model of sexually transmitted infection. Pigs were mock infected, infected once or infected and re-infected intravaginally and samples were obtained for chlamydial culture, gross and microscopic pathology and humoral and cell-mediated immunity. Intravaginal inoculation of pigs with this bacterium resulted in an infection that was confined to the urogenital tract, where inflammation and pathology were caused that resembled what is seen in human infection. Re-infection resulted in more severe gross pathology than primary infection and chlamydial colonization of the urogenital tract was similar for primary infected and re-infected pigs. This indicates that primary infection failed to induce protective immune responses against re-infection. Indeed, the proliferative responses of mononuclear cells from blood and lymphoid tissues to C. trachomatis strain L2c were never statistically different among groups, suggesting that C. trachomatis specific lymphocytes were not generated following infection or re-infection. Nevertheless, antichlamydial antibodies were elicited in sera and vaginal secretions after primary infection and re-infection clearly demonstrating a secondary systemic and mucosal antibody response. The porcine model is relevant for evaluating immune and pathogenic responses for emerging and known C. trachomatis strains to advance drug and/or vaccine development in humans.
Daisy Vanrompay is the Director of the Laboratory for Immunology and Animal Biotechnology at Ghent University and Director of the Belgian National Diagnostic Reference Laboratory for C. psittaci infections in humans and she is also a Member of PROVAXS.
Email: Daisy.Vanrompay@ugent.be