ISSN: 2471-9315
Cheong Ung Kim, Chan Hee Lee, Jae Hee Kim, Young Kyung Park, Sook Shin, Kun Taek Park and Yong Ho Park
Seoul National University, Republic of Korea
Posters & Accepted Abstracts: Appli Micro
Antimicrobial Resistant (AR) Acinetobacter baumannii is one of the most life-threatening pathogens causing nosocomial infections. However, limited information is available for AR A. baumannii isolated from livestock. Contaminated raw milk could be a vehicle for transmission of several zoonotic pathogens from animals to humans. The purpose of this study was to evaluate the prevalence of A. baumanni in raw milk and characterize their antimicrobial susceptibility in Korea. A total of 490 raw milk samples were collected from 49 different dairy farms in 2017. A. baumannii was isolated using CHROMagarâ�?¢Acinetobacter following the manufacturerâ�?�?s instruction and confirmed by a target specific PCR method. Antimicrobial susceptibility test was performed by a standard disc diffusion method against 14 different antimicrobials (7 different classes), according to the guidelines of Clinical Laboratory Standards Institute (CLSI, 2016). Of 490 raw milk samples; 136 samples were contaminated with A. baumannii (27.8%). Antimicrobial susceptibility test revealed that 60 of the 136 isolates (44.1%) were AR A. baumannii showing resistance against at least one antimicrobial. The highest AR rate was found against tetracycline (30.8%), followed by cefotaxime (12.5%), ceftriaxone (4.4%) and gentamicin (2.9%). Only one isolate was determined as multidrug resistant A. baumannii. However, no resistance was found to imipenem, meropenem and levofloxacin in all the isolates. In comparison with a previous study done in Korea, the current study indicates that the overall AR rate of A. baumannii in raw milk has significantly increased in the past 5 years, especially against tetracycline (5.3% to 30.8%) and cefotaxime (0% to 12.5%). In conclusion, our results suggest that the increased prevalence of AR A. baumannii in raw milk in Korea. Therefore, further studies may be needed to investigate the effect of AR A. baumannii in raw milk on public health due to the possible transmission of them.
Cheong Ung Kim has his expertise in veterinary microbiology and passion in research. His significant area of interest is multi-drug resistant (MDR) A. buamannii. He has obtained DVM at KonKuk University. He is currently pursuing PhD in Department of Veterinary Microbiology at Seoul National University, South Korea.