Polarity directed extraction optimization, total phenolic-flavono | 43387
Medicinal & Aromatic Plants

Medicinal & Aromatic Plants
Open Access

ISSN: 2167-0412

+44 1300 500008

Polarity directed extraction optimization, total phenolic-flavonoid content, HPLC-DAD analysis, antioxidant, antimicrobial, cytotoxic and anti-leishmanial potentials of Atriplex lasiantha Boiss

Joint Event on 4th World Congress on Medicinal Plants & Natural Products Research and 12th Global Ethnomedicine & Ethnopharmacology Conference

August 08-09, 2018 Osaka, Japan

Tanzeel Zohra

Quaid-i-Azam University, Pakistan

Posters & Accepted Abstracts: Medicinal & Aromatic Plants

Abstract :

Plants represent an intricate and innovative source for the discovery of novel therapeutic mediators for the management of various ailments. This study was aimed to validate the therapeutic potential of ethno medicinally significant plants Atriplex lasiantha Boiss. The polarity based extraction process was carried out using fourteen solvents to optimize best extraction solvent and bioactive fractions. Total phenolic-flavonoids contents were quantified colorimetrically and polyphenolics were measured using HPLC-DAD analysis. Further, the test samples were tested against several diseases targets including free radicals scavenging, antibacterial, antifungal, cytotoxic and anti-leishmanial potentials following standard procedures. Among the solvent fractions, maximum yield was obtained with methanol-water extract i.e., 11±0.49%. Maximum quantity of gallic acid equivalent phenolic content and quercetin equivalent flavonoid content were quantified in methanol-ethyl acetate extract of A. lasiantha aerial part. Significant quantity of rutin i.e., 0.3 μg/mg was quantified by HPLC analysis. The methanol-ethyl acetate extract of aerial part of A. lasiantha exhibited maximum total antioxidant and total reducing power with 64.8±1.16 AAE/mg extract, respectively, while showing 59.8±1.07% free radical scavenging potential. A significant antibacterial potential was exhibited by acetone-distilled water extract of aerial part of A. lasiantha with 11±0.65 mm zone of inhibition against B. subtilis. A remarkable antifungal activity manifested by ethyl acetate-n-hexane extract of aerial part of A. lasiantha with 14±1.94 mm zone of inhibition against A. fumigatus. Highest percentage of α-amylase inhibition potential (41.8±1.09%) was observed in ethyl acetate-n-hexane extract of A. lasiantha aerial part. Methanol-acetone extract of A. lasiantha demonstrated remarkable inhibition of hyphae formation with 11±0.49 mm bald zone of inhibition. Significant in vitro cytotoxicity against Hep G2 cell line was displayed by methanol-chloroforms extract of aerial part of A. lasiantha. The current study reveals the prospective potential of a novel plant Atriplex lasiantha Boiss. for the discovery of biologically active compounds through bioassay guided isolation in various diseases.

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