Dileep Kumar B S
CSIR - National Institute for Interdisciplinary Science and Technology, India
Posters & Accepted Abstracts: Appli Micro Open Access
Rhizosphere, rhizoplane and endophytic bacterial strains were isolated from different parts of India with a special reference to the state of Assam, and Kerala. Tea (Camellia sinensis (L.) Kuntze), Pigeon pea (Cajanus cajan (L.) Millsp.), rice (Oryza sativa L) and virgin forest soils were selected for the isolation of the organisms. The screening and selection were done on the basis of their in vitro antagonism against common plant pathogens. The strains exhibited antagonism were selected for their ability to promote plant growth and disease control under gnotobiotic condition with tea, pigeon pea and rice. The strain designated as Pseudomonas fluorescens RRLJ 134, Pseudomonas aeruginosa RRLJ 04, Bacillus cereus PM 43, Pseudomonas aeruginosa PM 105, and Pseudomonas species PM 112 (for tea), Pseudomonas aeruginosa RRLJ 04, Bacillus cereus BS 03 (for pigeon pea) Paenibacillus elgii NIIST B 578, Bacillus subtilis NIIST B 580 and Burkholderia gladioli NIIST B 567 (for rice) were selected for further studies under nursery and field condition. The experimental results confirmed that the bacterial treated plants exhibited an enhanced plant growth in terms of the number of new leaves with more chlorophyll content, number of lateral branches, shoot height and root length. RRLJ 134 and RRL 04 induced systemic resistance in tea plants against brown root rot and charcoal stump rot caused by Fomes lamoensis and Ustulina zonata, respectively and BS 03 and RRLJ 04 against wilt disease caused by Fusarium udum in pigeon pea. The resistance attained plants showed an increased level of defense related enzymes such as L-phenylalanine ammonia lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO) and total phenol. Endophytic strains (NIIST B 578, NIIST B 580 and NIIST B 567) improved growth and yield in rice and suppressed the sheath blight disease caused by Rhizoctonia solani.
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