Immunome Research
Open Access
ISSN: 1745-7580
ISSN: 1745-7580
Sidra Islam and Moinuddin
J N Medical College- AMU, India
Posters & Accepted Abstracts: J Immunome Res
Methylglyoxal (MG), a highly reactive endogenous �?±-oxoaldehyde is a deceptively small molecule formed during the metabolism of biological macromolecules. It is an intermediate of Maillard reaction and is a potent glycating agent showing 20,000 times more glycating potential than glucose. It reacts rapidly with proteins forming hydroimidazolone derivatives, hence, making them highly immunogenic. Increased protein-MG adducts have been reported in various pathological states such as diabetes and rheumatoid arthritis. In the present study, MG mediated structural alterations in IgG were characterized by various physicochemical techniques. Further, the immunogenicity of native and MG treated IgG was probed in female rabbits. The results revealed hyperchromicity in UV spectroscopy and AGE specific fluorescence, quenching in tyrosine fluorescence, tertiary structural change in near-UV CD, increase in hydrodynamic radii in DLS, aggregate formation in thioflavin T and Congo red staining assay and increase in molecular mass in MALDI-TOF MS. The modified IgG was highly immunogenic inducing high titer antibodies. From the results, it can be concluded that MG modification of IgG generate neo-epitopes on the molecule making it immunogenic. The results point towards a possible role of MG-IgG in the complications associated with diabetes type 2.
Email: sidraa.islam11@gmail.com