PHoP mediated Colistin-Resistance in Enterobacteria in water in t | 61060

Applied Microbiology: Open Access
Open Access

ISSN: 2471-9315

PHoP mediated Colistin-Resistance in Enterobacteria in water in the Ho Municipality, Ghana

10th World Congress and Expo on Applied Microbiology

November 21-22, 2022 | Webinar

E. U. Osisiogu*, K. O. Duedu and P. K. Feglo

Dr Hilla Limann Technical University, Ghana
Kwame Nkrumah University of Science and Technology, Ghana
University of Health and Allied Sciences, Ghana

Scientific Tracks Abstracts: Appli Microbiol Open Access

Abstract :

The advent and spread of colistin resistance genes have significantly hampered the efficacy of colistin; which is a last resort antibiotic for treating terminal infections. The rising prevalence of AMR in Gram-negative bacteria is a major medical concern with ramifications that will inevitably affect a variety of socioeconomic aspects on a global scale. Globally, both humans and animals are colonized by potentially pathogenic colistinresistant organisms and this has left the world with limited therapeutic options. Thus, this study sought to determine the relationship between the type of habitat and the presence of bacteria with colistin resistance genes. A total of 39 water samples were collected aseptically from five (5) different communities in the Volta region of Ghana from June 2021 to August 2021. Bacteria was isolated from the samples and analysed for colistin resistance phenotypically using the MIC method. Isolates which showed resistance phenotypically were subjected to Whole Genome Sequencing (WGS) using the Oxford Nanopore Technology. The WGS data was processed and analyzed using the Epi2Me Cloud platform. No Mobile Colistin Resistance (MCR) gene was detected. However, PhoP which is part of a two-component regulating system that activates the PmrA/B system which has downstream effects, leading to antimicrobial resistance to colistin was found in 81.01% of the isolates. This gene was detected majorly in Lokoe and haboured mostly by Proteus mirabilis (37.84%). Some other Enterobacteria identified during the study to habour the PHoP gene include E. coli (16.22%), Klebsiella pneumoniae (5.41%), Pseudomonas aureginosa (16.22%) and Citrobacter werkmanii (5.41%). The results demonstrate a high frequency of colistin resistance in enterobacterial strains isolated from water samples in the region. Thus, there is the need to halt the indiscriminate and non-therapeutic use of colistin to prevent the emergence and spread of colistin resistance genes. Keywords: PHoP gene, Colistin resistance, Enterobacteria.

Biography :

Emmanuel Osisiogu is affiliated to the Department of Science Laboratory Technology, Faculty of Applied Science and Technology, Dr Hilla Limann Technical University, Wa, Ghana.