Journal of Drug Metabolism & Toxicology
Open Access

Pharmacokinetics/pharmacodynamics modeling of cyadox against Clostridium perfringens in pigs

Joint Event on 31^st Euro Global Summit and Expo on Vaccines & Vaccination & 4^th World Congress and Exhibition on Antibiotics and Antibiotic Resistance

June 14-16, 2018 Barcelona, Spain

Lingli Huang, Lei Yan, Shuyu Xie, Dongmei Chen, Yuanhu Pan, Yanfei Tao, Wei Qu, ZhenLi Liu and Zonghui Yuan

Huazhong Agricultural University, China

Posters & Accepted Abstracts: J Drug Metab Toxicol

Abstract :

The objective of this study was to evaluate the antibacterial activity of cyadox against 60 pig C. perfringens and optimize the dosage regimens using ex vivo pharmacokinetics-pharmacodynamics (PK-PD) modeling. The ileum fluid contained free fraction cyadox of pigs after oral administration of 30 mg/kg body weight were collected by utilizing the implanted ultrafiltration probes. The Cmax of free cyadox in the ileum fluid was 23.66 �?¼g/mL and significantly higher than total concentration of 0.031 �?¼g/mL in plasma. The mean values of Tmax, AUC24h, and CL/F in ileum fluid were 1.96 h, 106.40 �?¼gâ�?�?h/mL, and 0.27 L/kg/h, respectively. The minimum inhibitory concentration (MIC) of cyadox against 60 clinical isolates ranged from 0.5 to 8 �?¼g/mL with MIC50 and MIC90 of 2 and 4 �?¼g/mL. The MIC of cyadox against pathogenic Clostridium perfringens CPFK122995 was 2 �?¼g/ mL in broth and ileum fluids. Cyadox displayed a concentration-dependent kill action with mutant prevention concentration (MPC) of 12 �?¼g/mL. According to the inhibitory sigmoid Emax modeling, the AUC24h/MIC ratios of ileum fluid required to achieve the bacteriostatic, bactericidal, and virtual bacterial elimination effect were 26.72, 39.54, and 50.69 h, respectively. Monte Carlo simulations for the 90% target attainment rate (TAR) predicted population daily doses to achieve bacteriostatic, bactericidal, and elimination actions over 24 h were 29.30, 42.56, and 54.50 mg/kg, respectively. These results suggested cyadox is a promising antibacterial agent to treat C. perfringens infections and provided a sound guidance for the usage.

Biography :

Lingli Huang is a Professor in the Department of Veterinary Medicine, and a research member in National Reference Laboratory of Veterinary Drug Residue at Huazhong Agriculture University in China. She received PhD in Agricultural Products Processing and Storage from Huazhong Agriculture University in 2005. Her current research aims at pharmacokinetics, metabolism and food safety evaluation of veterinary drug in food animals. She has published more than 45 papers in reputed international journals.
Email:huanglingli@mail.hzau.edu.cn