Journal of Antivirals & Antiretrovirals
Open Access

Panton – valentine leukocidin producing methicillin resistant Staphylococcus aureus from three health care centers in Tabuk city, Saudi Arabia

Joint Event on 4^th International Conference on Antimicrobials, Multiple Drug Resistance & Antibiotics Resistance & 4^thInternational Conference on Antibiotics R&D, B2B

April 20-21, 2018 Las Vegas, USA

Bernard C Silvala and Faisel M Abu-Duhier

University of Tabuk, Saudi Arabia

Scientific Tracks Abstracts: J Antivir Antiretrovir

Abstract :

Panton-valentine leukocidin (PV-luk) toxin is a very potent toxin responsible for severe and fatal form of Staphylococcal infections. This study aimed to determine the frequency of PV-luk producing methicillin resistant S. aureus (MRSA) and compare the conventional against real time PCR for identification of MRSA. A total number of 47 MRSA isolates were enrolled in the study. All are identified and typed using VITEK system. DNA was extracted and subject to conventional and real time PCR (RT-PCR) for the detection of mecA, luk-PV, orfX, mecA/mecC and SCC/orfX junction genes. Forty-five isolates (95.7%) confirmed as MRSA and two isolated showed a mixed infection of methicillin sensitive S. aureus (MSSA) and methicillin resistant coagulase negative Staphylococci (MRCoNS). Fifteen out of forty-seven (31.9%) have Panton ��? valentine leukocidin gene (luk-PV) gene. PV-luk was detected in one third of tested MRSA emerging the possibility to have a potent superbug. In addition to, diagnosis of MRSA is highly accurate when using multiplex real time PCR rather than conventional PCR.

Biography :

Bernard C Silvala is the current Department Head of the Medical Laboratory Technology Program at the University of Tabuk. He is an Assistant Professor in Microbiology. He has published numerous works in microbiological assays, educational manuals and scientific articles. Also, a member of Editorial Board Reviewer of reputable journals.
Email:bsilvala@ut.edu.sa