Journal of Clinical and Cellular Immunology
Open Access

Oral tolerance induction by brown spider recombinant toxins (wild-type and mutated) and its effects on immune response

2^nd International Conference on Clinical & Cellular Immunology

October 15-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Giovani Marino Favero, Murilo Delgobo, K�tia Sabrina Paludo, Ana Carolina Martins Wille and Silvio Sanches Veiga

Posters: J Clin Cell Immunol

Abstract :

Oral tolerance refers to physiologic induction of tolerance that occurs in the gut associated lymphoid tissues (GALT) and more broadly at other mucosal surfaces such as the respiratory tract. In the present work we proposed an oral tolerance protocol in adult Swiss miceby oral administration of a recombinant dermonecrotic toxin of brown spider Loxoscelesintermedia (LiRecDT1) and its mutated form(LiRecDT1H12A) for three weeks. At the end of protocol, animals were immunized with mutated toxin and mineral oil as adjuvant. At the fourteenth day, animals were boosted with mutated toxin and serum was collected seven days later. To access the induction of oral tolerance, specific levels of IgG anti-dermonecrotic toxin were measured via Enzyme linked immunosorbent assay (ELISA) in tolerized and immunized animals. Paw edema was performed in tolerized and control groups by injecting 6 μg of dermonecrotic toxin subcutaneously in the plantar surface. After ten minutes, paw edema was measured with micrometer and result was expressed in millimeter.Mice mortality study was developed by injecting 50 μg of dermonecrotic toxin intraperitoneally in control group and tolerized animals. Mice were observed for 8, 16 and 24h after injection. Our results demonstratedevidences of tolerance induction through decrease in IgG anti-dermonecrotic toxinlevels,paw edema reduction and increased survey in 24h after challenge. All statistical analysis was performed using ANOVA following Bonferroni?s pos hoc test. The study was sponsored by grants of Funda��o Arauc�ria, CAPES and CNPq.

Biography :

The characteristic of the research group is multidisciplinarity. The group has carried out research in the area of pathophysiology, pharmacology, involving principally immunology. Most of researchers involved have a consistent publication of papers in international journals and project approvals in Funding Agencies. Giovani Marino Favero was selected for presentation of this work.