Immunome Research
Open Access
ISSN: 1745-7580
+44-20-4587-4809
ISSN: 1745-7580
+44-20-4587-4809
Mazhar I Khan, Jianping Li, Zeinab H Helal, Christopher P Karch, Neha Mishra, Theodore Girshick, Antonio Garmendia and Peter Burkhard
University of Connecticut, USA
Posters & Accepted Abstracts: Immunome Res
Infectious bronchitis virus (IBV) affects poultry respiratory, renal and reproductive systems. Currently, the efficacy of available live attenuated or killed vaccines against IBV is been challenged. We designed a novel IBV vaccine alternative using a highly innovative platform called self-assembling protein nanoparticle (SAPN). In this vaccine, B cell epitopes derived from the second heptad repeat (HR2) region of IBV spike proteins repetitively presented in its native trimeric conformation. In addition, flagellin was co-displayed in the SAPN to achieve a self-adjuvanted effect. Three groups of chickens were immunized at four weeks of age with the vaccine prototype, IBVFlagellin- SAPN, a negative-control construct Flagellin-SAPN or a buffer control. The immunized chickens were challenged with 5x104.7 EID50 IBV M41 strain. High antibody responses were detected in chickens immunized with IBV-Flagellin-SAPN. In ex vivo proliferation tests, peripheral mononuclear cells (PBMCs) derived from IBV-Flagellin-SAPN immunized chickens had a significantly higher stimulation index than that of PBMCs from chickens receiving Flagellin-SAPN. Chickens immunized with IBV-Flagellin-SAPN had a significant reduction of tracheal virus shedding and lesser tracheal lesion scores than did negative control chickens. The data demonstrated that the IBV-Flagellin-SAPN holds promise as a vaccine for IBV.