Journal of Glycomics & Lipidomics
Open Access

Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: Overexpression of endogenous Δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated f

2^nd International Conference and Expo on Lipids: Metabolism, Nutrition & Health

October 03-05, 2016 Orlando, USA

Yuval Kaye

Carnegie Institution for Science, USA

Posters & Accepted Abstracts: J Glycomics Lipidomics

Abstract :

Nannochloropsis oceanica is an important source for omega-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), and a potent candidate for biofuel production, due to its outstanding capability for rapid induction of triacylglycerol (TAG) overproduction. In contrast to membrane lipids, TAG of N. oceanica is poor in the valuable LC-PUFA, EPA. We identified, cloned and characterized a N. oceanica microsomal-like �?�?12-desaturase (NoD12) mediating the committing step of LC-PUFA biosynthesis by converting oleic acid (18:1 n-9) to linoleic acid (LA, 18:2 n-6). We generated strains of N. oceanica over expressing NoD12 under the control of the stress-inducible endogenous lipid droplet surface protein (LDSP) promoter, resulting in robust expression under nitrogen starvation conditions. The overexpression of NoD12 significantly altered fatty acid composition of total lipids and of individual lipid classes, such as a drastic increase in 18:2 proportion in phosphatidylcholine and in TAG was observed under nitrogen starvation. Some LA was converted further toward LC-PUFA resulting in a substantial increase in arachidonic acid (20:4 n-6) in TAG. Our data demonstrate the feasibility of metabolic engineering to increase LC-PUFA content in the biotechnologically important microalgae using native genes and promoters, and provide novel insights into the regulation of LC-PUFA flux to TAG under nitrogen starvation.

Biography :

Email: ykaye@carnegiescience.edu