Investigation of IL-12B gene polymorphism (rs3212227) in Iranian | 56639
Journal of Clinical and Cellular Immunology

Journal of Clinical and Cellular Immunology
Open Access

ISSN: 2155-9899

Investigation of IL-12B gene polymorphism (rs3212227) in Iranian patients with alopecia areata

2nd International Conference on Autoimmunity

November 06-07, 2017 | Frankfurt, Germany

Reyhaneh Abgoon, Sanaz Sepehri, Reza Akbarzadeh and Akram Sadat Tabatabaei Panah

Islamic Azad University, East Tehran Branch, Iran
Shahid Beheshti University of Medical Sciences, Iran

Scientific Tracks Abstracts: J Clin Cell Immunol

Abstract :

Objective: Alopecia areata (AA) is an autoimmune disease characterized by patchy hair loss affecting both scalp and body hair. Although the etiology and pathogenesis of this disease are still unknown, a polymorphism within IL-12B gene has been described in few studies to be associated with AA susceptibility. Yet, these findings had so far not been independently replicated, and no data on a possible association of IL-12B mutation and AA in Iranian population were available. Methods: This study contains 30 AA patients and 15 healthy controls. Genomic DNA was isolated using DNG-plus and PCRRFLP analysis was performed to detect IL-12B rs3212227 polymorphism. Several relevant information such as demographic data (age, gender) or clinical characteristics were analyzed for a possible effect of these factors on susceptibility to AA in patients who carry CC, AC, and AA genotypes. Results: No association between the IL-12B rs3212227 mutation and susceptibility to AA was observed in our Iranian cohort. PCR-RFLP results showed that frequency of CC genotype (13.3% vs. 6.6%) are similar in both patient and control groups. AC genotype was detected in 46.6% and 6.6% of patients and controls, respectively. The AA genotype which is wild genotype had a higher frequency in healthy individuals. Statistical analyses indicate that there is no significant difference in the distribution of genotypes between patients and controls (P=0.12). Although the C allele frequency of IL-12B was higher in the patients than control subjects (36.6% vs. 10% respectively), but there is no significant difference (P=0.12). Conclusion: We here demonstrate that the IL-12B rs3212227 polymorphism is not associated with the risk to develop AA in our Iranian cohort. Therefore, this study failed to confirm a reported association between gene mutation and susceptibility to AA. Hence, the genetic predisposition to develop AA greatly varies among different ethnic groups.? 

Biography :

Reyhaneh Abgoon has received her Master’s degree in Cellular and Molecular Biology from Azad University. Her research interests include Immunology, Molecular Immunology, especially Autoimmune Diseases. She is working as a Supervisor in Banej Elixir molecular research institute in Tehran. She has presented several research abstracts about alopecia areata which is an autoimmune disease at various international conferences.