Neslihan Sursal, Ayse Cakmak and Kader Yildiz
Ankara University, Turkey
Kirikkale University, Turkey
Posters & Accepted Abstracts: J Clin Cell Immunol
This work was supported by the grand from TUBITAK (Project no: TOVAG 214O288)
Neutrophils are the most important host innate immune defenders which freely circulate in blood and recruited to inflammation area. Neutrophils are short lived polymorphonucleargranulocytes (PMN) and play an important role activity to the inflammation area in vertebra hosts and they kill the pathogens with various defence strategies (phagocytosis, degranulation and netosis). During netosis, the neutrophil nuclear and granule contents are given to extracellular region to fight with pathogens. It is reported that neutrophil extracellular traps (NETs) exist in several bacteria, fungi, viruses and protozoa. There are no information on netosis reaction to T.gondii in cat which are definite hosts and intermediate hosts of toxoplasmosis. Hereby, it is intended to demonstrate NETs reaction in cat├?┬?├?┬ó├?┬?├?┬?├?┬?├?┬?s neutrophils confronted with T.gondii tachyzoites in vitro. Whole blood of cat layered with Percoll solutions(72, 63, 54 and 45%) for neutrophil purification. Then, NETs activitywas observed using fluorescence microscopy. Myeloperoxidase, neutrophil elastase and histone (H3) shown as co-localise in fine DNA networks released from cat PMN after reacting with T.gondii tachyzoites. The results suggest cat PMN create NETs to T.gondii tachyzoites and netosis is important mechanism for fighting with T.gondii in cats. According to Authors knowledge, it is the first report that NETsdevelop in cat neutrophils against to T.gondii tachyzoites.
Neslihan Sursal has completed her PhD education from Ankara University, Turkey