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Improvement and application of nested-PCR technique using consensus-degenerate primers to detect human herpes virus in plasma samples from hematopoietic stem cell transplantation (HSCT) patients
Journal of Antivirals & Antiretrovirals

Journal of Antivirals & Antiretrovirals
Open Access

ISSN: 1948-5964

Improvement and application of nested-PCR technique using consensus-degenerate primers to detect human herpes virus in plasma samples from hematopoietic stem cell transplantation (HSCT) patients


5th World Congress on Virology

December 07-09, 2015 Atlanta, USA

Talita Cristina Dellariva

State University of Campinas, Brazil

Posters-Accepted Abstracts: J Antivir Antiretrovir

Abstract :

Human herpes viruses (HHVs) are an important cause of infections in transplantation and immunocompromised patients in general. This group of virus consists in three subfamilies: ?±-herpesvirinae, ?²â??herpesvirinae and ?³â??herpesvirinae which include the eight human herpes virus species. Currently, the diagnosis of infectious diseases have been a considerable advance after molecular techniques implantation, especially the polymerase chain reation (PCR), once it presents high sensitivity and specificity to detect low quantities of nucleic acids. The use of consensus-degenerate primers in PCR has been used as an efficient alternative for the detection and identification of Herpes viridae family members in clinical samples. The aim of this study was to improve the nested-PCR (N-PCR) technique using consensus-degenerate primers to detect human herpes virus sub-families. Forty plasma-DNA samples from HSCT patients were analyzed using N-PCR technique with degenerate primers in both reactions. These primers were designed from highly conserved amino acid sequences of herpesviral DNA polymerase gene. The results show that 8/40 (20%) were positive to ?±-herpesvirinae and/or ?³â??herpesvirinae; 19/40 (47.5%) were positive to ?²â??herpesvirinae and 5/40 (12.5%) presented coinfection with the three subfamilies. The development and improvement of N-PCR using consensus-degenerate primers have been fundamentally important in helping the clinical diagnosis, therapy, classification and epidemiological studies of viruses and to discover new herpesvirus species.

Biography :

Talita Cristina Dellariva completed her degree in Biological Sciences from State University of Campinas (UNICAMP) in 2013. She has been working on her Master’s degree in Clinical Medicine in the Faculty of Medical Sciences, State University of Campinas, São Paulo, Brazil.

Email: [email protected]

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