Journal of Clinical and Cellular Immunology
Open Access

Immune enhancing activity of Cordyceps militaris fermented with probiotics in vitro and in vivo

Conference Series LLC Joint International Event on 5^th European Immunology & Innate Immunity

July 21-23, 2016 Berlin, Germany

Hye-Jin Park and Woo-Ri Jo

Gachon University, South Korea

Posters & Accepted Abstracts: J Clin Cell Immunol

Abstract :

The goal of the present study was to investigate the immune-enhancing activity of C. militaris grown on germinated soybeans (GSC) fermented with Pediococcus pentosaceus (ON89A) (GSC-ON89A). We previously reported that the polysaccharides from GSC have immunostimulatory activities in RAW 264.7 macrophages and anti-viral activities. In this study, the immunostimulatory activities of GSC-ON89A were investigated further using various experimental models such as in vitro, ex vivo and in vivo. Among GSC fermented with probiotics (Pediococcus pentosaceus (SC11), Lactobacillus pentosus (SC64), Weisslela cibaria (spum8), Lactococcus lactis (Bro17), Pediococcus pentosaceus (SO-024), Pediococcus pentosaceus (ON89A), Pediococcus pentosaceus (ON188), Lactobacillus sakei (sal.cla22), Weissella cibaria (Bro22), Pediococcus pentosaceus (GO088)), GSC-ON89A induced the highest elevation of nitric oxide (NO) production in RAW264.7 cells. In addition, GSC-ON89A showed higher phagocytic activity than control or GSC in RAW264.7 cells. In primary cultured mouse peritoneal macrophages, the GSC-ON89A was found to significantly increase phagocytic activity, compared to control and GSC. In the normal mice model, the oral administration of GSC-ON89A improved the NO production of peritoneal macrophages, compared to control and GSC. These results indicate that GSC-ON89A stimulates the immune response of macrophage in vitro and in vivo.