Scientific Tracks Abstracts: J Clin Cell Immunol
IL-2 is one of the most extensively used adjuvants for vaccination to stimulate the proliferation and differentiation of effector T cells. Follicular helper T cells (Tfh cells) are a new CD4+T cell subset that specializes in helping formation of germinal center (GC) and, consequently, mediated the T-dependent humoral immunity response. However, it is uncertain whether IL-2 as an adjuvant may modulate the Tfh cells immune response. In this study, we investigated the effects of IL-2 adjuvant for adenovirus-vectored FMD vaccine on production of FMDV VP1 specific IgG, IgG1 and IgG2a, secretion of IFN-γ, IL-4 and IL-21 in serum, expression of Bcl-6 mRNA from mice after immunization. Further, the generation of Tfh cells, GC B cells and formation of GC from mice after immunization were explored. The data showed that IL-2 as an adjuvant for adenovirus- vectored FMD vaccineenhanced both levels of antibodies and secretion of IFN-γ,IL-4 and IL-21 in serum, which revealed the potent adjuvant activity of IL-2 could enhance the generation of Th1 (IFN-γ) and Th2 (IL-4), as well as Tfh (IL-21) cells. The further effects of IL-2 on Tfh cells were detected and showedthat IL-2 as an adjuvant for adenovirus-vectored FMD vaccine increased the generation of Tfh cells and the expression of Bcl-6 mRNA, additionally, IL-2 as an adjuvant for adenovirus- vectored FMD vaccine substantially increased the generation of GC B cells and formation of GCs, which revealed that IL-2 as an adjuvant for vaccination induced generation of Tfh cells and then mediated T-dependent humoral immunity response.
Chunxia Su has completed her PhD at the age of 30 years from Nanjing Agriculture University. She has published more than 10 papers in reputed journals.