Xiaosong Chen, Zhihui Guo, Ming Li, Chushan Huang, LiuYan and Ying Chen
Union Hospital of Fujian Medical University, China
Posters & Accepted Abstracts: Orthop Muscular Syst
Objective: This study is to identify whether miR-26a is a target gene of bile acid receptor TGR5 in BAT in spontaneous and dietinduced obesity animal models, thus providing a potential link between TGR5 and miR-26a expression. Methods: We used spontaneous and diet-induced obesity mice to be determined whether TGR5 is the only receptor required to mediate the effect of OA on obesity. Mouse body weights were monitored during the entire process. Histological examination and metabolic measurementswere also performed. Results: TGR5 partially mediated the effect of OA on obesity and glucose regulation.TGR5 activation increased the expression of miR-26a.TGR5 activation up-regulated miR-26a expression in macrophages.JNK pathway was downstream of TGR5 in miR-26a induction.TGR5 responsible DNA sequences were in the proximal regions of miR-26a promoter. Conclusion: These findings suggest the activation of TGR5 by OA can modulate the expression of miR-26a through a JNK-dependent pathway for the treatment of obesity-associated metabolic diseases.