National Cheng Kung University, Taiwan
Posters & Accepted Abstracts: J Clin Cell Immunol
Background: Acanthamoeba keratitis (AK) is a serious disease caused by pathogenic Acanthamoeba through wounds
of cornea; by and large, most high-risk patients catching AK wear contact lens generally over a long period of time.
However, microscopic examination and polymerase chain reaction following the culture that they are traditional
diagnostic tests for pathogenic Acanthamoeba need long time to confirm AK. Hence, we purpose to develop a rapid
and sensitive antibody-based assay to improve the diagnostic procedure.
Objective: Comparing with proteomic analysis of extracellular secreted proteins expressed by two pathogenic Acanthamoeba castellanii clinical isolates and a non-pathogenic ATCC strain, we will evaluate the efficiency of Acanthamoeba M28 aminopeptidase (M28–AAP) as a diagnostic target for developing a rapid and sensitive antibodybased assay and investigate molecular and characteristics of the M28–AAP.
Methods: The M28–AAP antibody was produced to detect Acanthamoeba isolates through an antibody-based diagnostic test such as a western blot or an enzyme-linked immunosorbent assay (ELISA).
Results: We have produced successfully M28–AAP antibody to detect Acanthamoeba isolates through a western blot, an immunofluorescence assay (IFA) and an ELISA. Furthermore, the complement can be degraded by M28–AAP in the human innate immunity. We believe that M28–AAP will be the opportunity to develop a rapid diagnostic marker and newer target to decrease the AK patient cases. To evaluate the sensitivity and specificity of the Acanthamoeba M28–AAP antibody, we will plan to develop rapid diagnostic tests such as test strips on clinical diagnosis.
Jian-Ming Huang is currently studying in National Cheng Kung University. His major project is to study the protein expression in the parasitic protozoan Acanthamoeba. He also developed antibody to identify a rapid and sensitive antibody-based assay and investigate molecular and characteristics of the M28_ AAP. A total of 2 papers were published by him based on his worke.
E-mail: [email protected]