Journal of Antivirals & Antiretrovirals
Open Access
ISSN: 1948-5964
+44 1300 500008
ISSN: 1948-5964
+44 1300 500008
Wenchao Sun, Changzhan Xie, Huijun Lu and Ningyi Jin
Chinese Academy of Agricultural Sciences, China
Posters & Accepted Abstracts: J Antivir Antiretrovir
Porcine reproductive and respiratory syndrome (PRRS) is the main cause of the reproductive disorders in gilts and sows. PRRS is considered one of the most economically important swine infectious diseases around the world. PRRSV is an enveloped positivestranded RNA virus which belongs to the family Arteriviridae. PRRSV has two distinct genotypes European type (type 1) and North American type (type 2). HP-PRRSV was classified into North American type; it has become the predominant circulating field strain in China from 2006 to till date. HP-PRRSV has a discontinuous 1+29 amino acid (aa) deletion in the NSP2 gene. The NSP2 gene is the one of most variable regions in the whole gene, although this discontinuous deletion has been proved not to the high virulence of HP-PRRSV, which reminds us that PRRSV is always mutations. ORF5 contains a neutralizing epitope, playing important roles in pathogenesis, including host cell entry and apoptosis and it is also a hypervariable region. The GXBB-11 strain was isolated from lung tissue in Guangxi, two pairs of primers amplifying NSP2 and ORF5. The PCR products were purified and cloned into pEASY-T1 vector (TransGen Biotech). Comparison of the GXBB-11 with the HP-PRRSV JXA1 by multiple alignment showed that 20 amino acid (aa) deletions (2566nt-2625nt) in the NSP2 region. Furthermore, the GXBB-11 showed 84.20% nucleotide homology with the ORF5 genome of the JXA1. The GXBB-11 genome sequence data could help us understand the evolutionary characteristics of HP-PRRSV in China.
Email: 13943184790@163.com