Journal of Antivirals & Antiretrovirals
Open Access
ISSN: 1948-5964
ISSN: 1948-5964
Adriele Fontes, Tatiane Assone, Karen Gaester, Rosa Marcusso, Alberto Duarte, Augusto C.P. Oliveira and Jorge Casseb
Poster Presentations: J Antivir Antiretrovir
Background: Human T-cell lymphotropic virus type 1 (HTLV-1) infection can increase the risk of developing neurological disorders. This study evaluated the correlation between HTLV-1 DNA proviral load among HTLV-1-infected individuals with HTLV-1-associated myelopathy (HAM/TSP). Material and Methods: In the last 15 years, one cohort of HTLV-infected subjects has been followed in the HTLV-outpatient Clinic at the Institute of Infectious Diseases ?Emilio Ribas? (IIER). The results of the first HTLV-1 proviral load were available in the database. Quantitative proviral DNA levels were detected by a real-time automated PCR method, using TaqMan probes for the pol gene. The albumin gene served as the internal genomic control, and MT2 cells were used as a positive control. The results are reported as copies/10000 PBMCs, and the detection limit was 10 copies. Results: From this cohort, 60 HAM/TSP patients were studied. In accordance to the DNA HTLV-1 proviral load results were divided in four intervals: < 10 copies/104 PBMC=; 12 (20%) patients; 11-50 copies/104 PBMC= 5 (8%); 101-500 copies/104 PBMC=; 22 (36%); and >501 copies/104 PBMC= 21 patients (35%) p≤0,001. We observed a significant increased in proviral load in patients with HAM/TSP, showing this parameter as a potential marker for disease progression.
Adriele S. Fontes was graduated in Biomedicine from the Integrated College Aparicio Carvalho, Porto Velho, Rondonia city. Actually is Master�s Student in Tropical Medicine and Health International at the IMTSP-USP.