High affinity anti-internalin B VHH antibody fragments isolated f | 4628
Immunome Research

Immunome Research
Open Access

ISSN: 1745-7580


High affinity anti-internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires

International Conference and Exhibtion on Antibodies

August 10-11, 2015 Birmingham, UK

Mehdi Arbabi

National Research Council, Canada

Posters-Accepted Abstracts: Immunome Res

Abstract :

The need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. Furthermore, distribution of tainted food and water can have consequences which can affect whole economies. Antibodies and antibody fragments have been historically used in detection platforms due to their antigen specificity and robust physicochemical properties. In this study, we report the isolation and characterization of antibody fragments from the immunized and non-immunized heavy chain antibody repertoire (VHH) of Camelidae which bind with specificity and high affinity to the Listeria monocytogenes invasin, Internalin B (InlB). To the best of our knowledge, this is the first report of anti- InlB VHHs from camelids. These anti-InlB VHHs were not cross-reactive to the structurally-related Listeria invasin Internalin A (InlA) and are potential reagents to be used in the development of detection and medical technologies.

Biography :