ISSN: 2471-9315
Maryam Ghayeb Zamharir, Mahdi Taheri, Kaveh Bananej, Abolghasem Ghasemi and Shokrolah Hajivand
Agricultural Research, Education & Extension, Iran
Takestan Jahadeh Keshavarzi Center, Iran
Posters & Accepted Abstracts: Appli Micro
Grape is one of the most valuable and the most important horticultural crops in Iran. Recently, phytoplasmas disease has been reported from these Qazvin and Lorestan provinces. Preliminary analysis shows that different strain associated with this disease. In order to study genetic diversity of phytoplasmas associate with grape decline using Tuf gene, different samples collected from these provinces. DNA extraction has been performed by CTAB method. PCR was employed to amplify Tuf gene in phytoplasmas using primer pair Tuf C/D. The expected 850 bp fragment of the phytoplasmal 16S rDNA was amplified. In order to study genetic diversity of grape phytoplasmas isolates, RFLP analysis has been performed on Tuf gene using EcorI, MseI and TaqI enzymes. Results of this study show that MseI enzyme could distinguish different phytoplasmas isolates in grape.
Maryam Ghayeb Zamharir has research interest in horticulture crops.