Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Weam A M Rashwan, Laila Rashed and Wael Sadek
Cairo University, Egypt
Posters & Accepted Abstracts: J Clin Cell Immunol
Background: Leukocyte Fc�?�?�?³ receptors for immunoglobulin G (Fc�?�?�?³Rs) play a major role in the handling of immune complexes and pathogens in Systemic Lupus Erythematosus (SLE) and periodontitis. Both diseases have been shown to be partly influenced by genetic components including Fc�?�?�?³R genotype. The aim of the present study was therefore to evaluate whether Fc�?�?�?³R gene polymorphisms are associated with periodontitis risk in Egyptian SLE patients. Methods: The study subjects consisted of 100 SLE patients with periodontitis (SLE+P), 100 SLE patients without periodontitis (SLE), 100 healthy subjects with periodontitis (P) and 100 healthy subjects without periodontitis (H), who were all unrelated Egyptian non smokers. Genomic DNA was isolated from peripheral blood and Fc�?�?�?³R genotypes for 3 biallelic polymorphisms (Fc�?�?�?³RIIa-R131/H131, Fc�?�?�?³RIIIa-158V/158F and Fc�?�?�?³RIIIb-NA1/NA2) were determined by PCR-RFLP and allele specific polymerase chain reactions. Results: The SLE+P group was found to have more levels of periodontal destruction than the P group (P<0.001). A significant over representation of the Fc�?�?�?³RIIa-R131 allele was found in the SLE+P group compared to the H group (SLE+P versus H: P<0.001, odds ratio, OR=10.553, 95% confidence interval, 95% CI=6.33-17.594). The Fc�?�?�?³RIIa-R131 allele was also found to be overrepresented in the SLE+P group compared to the SLE group (SLE+P versus SLE: P-value<0.001, OR=4.317, 95% CI=2.818- 6.613). The Fc�?�?�?³RIIa-R131 allele was also found to be overrepresented in the SLE+P group compared to the P group (SLE+P versus P, P-value=0.001, OR =1.987, 95% CI=1.335-2.958). The frequencies of Fc�?�?�?³RIIIa alleles among the subject groups showed SLE+Periodontitis group to have statistically significantly higher percentage of (F) allele than control group (SLE+Periodontitis vs. Control, VxF, P-value=0.001, OR=2.154, 95% CI=1.370-3.385). Same results were obtained when SLE+Periodontitis group was compared to periodontitis group (SLE+Periodontitis vs. periodontitis, VVxFF, P value<0.001, VxF, P value<0.001, OR=2.667, 95% CI=1.705-4.171). Same results were obtained when SLE+Periodontitis group was compared to SLE group (SLE+Periodontitis vs. SLE, VVxFF, P value=0.002). Same results were also obtained when SLE group was compared to control group (VVxFF, P value=0.030, VxF, P value=0.029, OR=1.615, 95% CI=1.048-2.489). Same results were also obtained when periodontitis group was compared to control group (VVxFF, P value<0.001). Conclusion: These results show the Fc�?�?�?³RIIA-R131, Fc�?�?�?³RIIIA-158F alleles to be associated with periodontitis risk in Egyptian SLE patients.