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Expression of microRNA-3148 and its correlation with copy number | 57380
Journal of Clinical and Cellular Immunology

Journal of Clinical and Cellular Immunology
Open Access

ISSN: 2155-9899

Expression of microRNA-3148 and its correlation with copy number variation of the TLR7 gene in Mayan women with systemic lupus erythematosus from Mexico


9th European Immunology Conference Associated with Antibody Engineering Meeting

June 14-16, 2018 | Rome, Italy

Guillermo Valencia Pacheco*, Yumi E. Nakazawa Ueji, Lizbeth J. Gonz?¡lez Herrera, Gerardo J. P?©rez Mendoza, Darig C?¡mara Cruz, Ang?©lica V. Angulo Ram?­rez, Ricardo F. L?³pez Villanueva and Irma G. Quintal Ortiz

Autonomous University of Yucatan, Mexico
Autonomous University of Yucatan, Mexico
Yucatan Health Services (SSY), Mexico.
ISSSTE Hospital Regional M?©rida, M?©xico

Posters & Accepted Abstracts: J Clin Cell Immunol

Abstract :

Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease characterized by autoantibody production. It is a multifactorial disease with a variety of clinical manifestations and it is found most often in women. Genetic factors have been associated with the development of SLE. Copy number variations (CNV) of genes as well as epigenetic factors such as microRNAs (miR) have been involved. The aim of this study was to correlate the expression of miR-3148 with CNV of TLR7 gene in Mayan women with systemic lupus erythematosus from Yucat?¡n. We studied 100 women diagnosed with SLE and 100 healthy women as a control group. The CNV and miR-3148, was determined by real-time PCR (Q-PCR) using TaqMan probes. The results indicate that 17% of the patients present more than two copies of the TLR7 gene, with respect to the controls. Correlation was found between CNV and TLR7 mRNA (r = 0.4080, n = 0.0016) and between miR-3148 and mRNA (r = 0.5950, p = 0.0002) in the patients, suggesting that both variables can be risk markers to develop the disease.

Biography :

Guillermo Valencia Pacheco has a PhD in Biomedical Sciences (Immunology). He is a Research Professor at the Research Center of the University of Yucatan and Head of the Hematology Laboratory. His line of research is in autoimmune diseases. He teaches Immunology at pre and postgraduate level. He has published more than 10 articles in indexed journals. He is a member of the Mexican Society of Immunology.

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