Rajendra Memorial Research Institute of Medical Sciences, India
Posters & Accepted Abstracts: J Clin Cell Immunol
Visceral leishmaniasis (VL) is a disease caused by protozoan species of the genus Leishmania and is transmitted
through bites from the Phlebotomus sand fly; it is associated with considerable morbidity and mortality in many
parts of world, including India. Reports on the protective role played by saliva proteins of Lutozomyia longipalpis,
Phlebotomus papatasi and Phlebotomus duboscqi are available. However, no studies have explored the salivary
proteins of P. +argentipes, which is the known proven vector for the transmission of VL in the Indian sub-continent.
Herein we revealed the presence of two proteins of 14.2 and one protein of 13.6 kDa in Indian strain P. argentipes
which is absolute identical to previously reported protein of SP15 family (PagSP01, PagSP02 and PagSP07) of P.
argentipes of NIH colony, USA. In an experimental study on P. argentipes from Bihar, India, we demonstrated that a
strong humoral and cellular immune response was triggered to reduce the concomitant Leishmania load in groups
of immunized mice. The immunized group produced a considerable amount of IgG antibodies, and their splenocytes
generated TH1 cytokines (IL-12, IFN-γ) with the support of delayed-type hypersensitivity (DTH) reactivity in such
mice at the challenged site. We summarize from our data that some identical proteins to previous from SP15 family
protein of 14.2 and 13.6 kDa molecular size, derived from Indian P. argentipes and reported its first time, can also be
significant in resolution of VL infection after modulation of host protective T cell response in VL.
1. Exploring new immunological insight on SP15 (~14kDa) family protein in saliva of Indian sand-fly (Phlebotomus argentipes) in experimental visceral leishmaniasis. (2018), Cellular immunology. 332, pp.51-57.
2. Cedrus deodara: In vitro anti-leishmanial efficacy & immumomodulatory activity. (2017), The Indian journal of medical research, 146(6), p.780.
3. Direct evidence for role of anti-saliva antibodies against salivary gland homogenate of P. argentipes in modulation of protective Th1-immune response against Leishmania donovani. (2016).Cytokine, 86, 79-86.
4. Degree of anemia correlates with increased utilization of heme by Leishmania donovani parasites in visceral leishmaniasis. (2013). Experimental parasitology. 135, 595-598.
5. Leishmania donovani vs. Immunity: T-cells sensitized from Leishmania of one donor may modulate their cytokines pattern on re-stimulation with Leishmania from different donor in visceral leishmaniasis: (2009). Experimental Parasitology 121, 69-75.
Shyam Narayan has more than 25 years research experience in the field of leishmaniasis to reveal the resistant isolates demography, their role in protective cytokine diversion, control of parasitic anemia in visceral leishmaniasis as well as identification of phytopharmaceutical anti-leishmanial candidate and also to identify the candidate for vector based vaccine development against leishmaniasis. His research papers are published in well reputed journals.
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