Evaluation of the agreement between comparative intra-dermal tube | 9044
Immunome Research

Immunome Research
Open Access

ISSN: 1745-7580


Evaluation of the agreement between comparative intra-dermal tuberculin and gamma interferon tests for the diagnosis of bovine tuberculosis in Ethiopia

8th Molecular Immunology and Immunogenetics Congress

March 20-21, 2017 Rome, Italy

Yewubdar Gulelat, Ketema Tafess, Aboma Zewde, Mulualem Ambaw, Markos Abebe, and Gobena Ameni

Dilla University, Ethiopia
Armauer Hansen Research Institute, Ethiopia
Jimma University, Ethiopia
Addis Ababa University, Ethiopia

Posters & Accepted Abstracts: J Immunome Res

Abstract :

The control of bovine tuberculosis is difficult in developing countries because of the high cost of a sustainable testing programs and the lack of a diagnostic assays with high sensitivity and specificity to detect animals at all stages of infection. A cross-sectional study was conducted to evaluate the test agreement between comparative intra-dermal tuberculin test (CIDT) and interferon gamma (INF-�?³) tests for the diagnosis of bovine tuberculosis (BTB) at Holeta, Ethiopia from December, 2012 to May, 2013. In this study, a total of 114 cattles were tested both by CIDT and INF-�?³ tests. The agreement between INF-�?³ (PPDB-PPDA) and CIDT tests was fair (k=0.288). On the other hand, a moderate agreement (k=0.44) was observed between INF-�?³ test (PPDB-PPDA) and INF-�?³ test (ESAT6/CFP10-Nil). The difference between reaction sizes at the bovine PPD injection site and the avian PPD injection site was significant (t test; P<0.001). While there was no significance difference (t test; P>0.05) in INF-�?³ response to bovine PPD and avian PPD. INF-�?³ response was stronger for ESAT6-CFP10 protein cocktails compared to PPD (B-A). Logistic regression analysis showed a significant association of animal age group with the responses of CIDT and INF�?³ tests. In conclusion, the fair agreement between the CIDT and INF-�?³ tests could indicate that the two tests are not completely overlapping and thus in addition to detecting a similar stage of the disease they also detect the different stages of the disease. Hence, it is advisable to use the two tests in combination.

Biography :