Danish Idrees and Samudrala Gourinath
Jawaharlal Nehru University, India
Posters & Accepted Abstracts: J Fundam Renewable Energy Appl
Carbonic anhydrase (CA) is an enzyme of great interest because of its potential industrial and clinical application. Recently, a new concept developed to capture CO2 from the atmosphere for biofuel production, which involves the use of immobilized CA that catalyses the reversible hydration of CO2 to HCO3. Due to enormous industrial and clinical application, engineered CA produced which is highly stable, which provides long-term stability. This engineered CA worked efficiently well at high temperature without losing their activity significantly. This captured CO2 through engineered CA could be used as a potential carbon source to produce lipids for the generation of biofuel. We developed six engineered CAs and measured their activity, stability, and kinetics. The engineered CAs are, (i) CA with 6 N-terminal histidine (His), (ii) CA with 6 C-terminal His, (iii) CA with 6 N-terminal and 6 C-terminal His, (iv) CA with N-terminal Cellulose binding domain (CBD), (v) CA with C-terminal CBD and (vi) CA with N and C-terminal CBD along with wild-type CA. Cost-efficient production of stable enzyme system is critical for the development of the economically feasible CA-based application. All these variants and wild-type enzymes studied for their structure and confirmation by using UV-absorption, circular dichroism, and fluorescence spectroscopy. We found that variants showing good activity and relatively more stability at a higher temperature. These engineered CAs will be used in the production of biofuel.