Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
+44 1478 350008
ISSN: 2329-9029
+44 1478 350008
Smita Purohit
The IIS University, India
Scientific Tracks Abstracts: J Plant Biochem Physiol
Cuminum cyminum L. is an important seed spice belonging to family Apiaceae. Production of cumin is limited due to limited genetic diversity and several biotic stresses. Hence, the present study aims at improving the regeneration of cumin in in vitro cultures. A regeneration medium was standardized comprising of MS supplemented with kinetin (0.5 mg/l). Effect of thiamine was studied on the morphogenic competence of the in vitro cultures. The levels of thiamine in the induction as well as proliferation medium highly influenced the shoot regeneration. Highest number of shoot buds per explant was obtained when the concentration of thiamine was twofold the normal MS level at both induction as well as proliferation stages. Shoots upto 2 cm or more in length were excised and inoculated on rooting medium i.e., MS medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA). Rooted plantlets were transferred to field conditions. Recent Publications 1. Purohit S and Agarwal M (2017) Mineral manipulation and Antioxidative studies in Carnation - Dianthus caryophyllus L. International Journal of Crop Science and Technology 3:1-8. 2. Yadav A, Joshi A, Kothari S L, Kachhwaha S and Purohit S (2017) Medicinal, nutritional and industrial applications of Salvia species: A revisit. International Journal of Pharmaceutical Science Review & Research 43(2):27-37 3. Agarwal M and Purohit S (2013) Overcoming hyperhydricity and profiling the affected proteins in micropropagated carnation. IIS University Journal of Science and Technology 2(1):32-37. 4. Agarwal M and Purohit S (2013) Changes in antioxidant enzymes activity during in vitro morphogenesis of carnation and the effect of antioxidants on plant regeneration. World Journal of Sciences and Technology 2(7):87-92 5. Purohit S and Kothari S L (2007) Direct somatic Embryogenesis from cotyledon and cotyledonary node explants in Bishop�??s weed- Trachyspermum ammi (L.) Sprague. In vitro cellular & developmental biology- Plant 43(2):154-158.
Smita Purohit, Associate Professor & Former Head, Department of Botany, The IIS University, Jaipur has her expertise in plant tissue culture, stress physiology, molecular biology, mineral manipulation and phytochemical studies. She has worked on various plant systems like Cuminum cyminum, Dianthus caryophyllus, Cissus quadrangularis, Salvia hispanica to name a few. She has also authored books in the field of Genetics and Plant Breeding and has supervised few doctoral and many MPhil candidates and has published many research papers in national and international journals of repute.
E-mail: smita.purohit@gmail.com