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DNA-poor space in Hodgkinandprime;s lymphoma nuclei | 17848
Journal of Hematology & Thromboembolic Diseases

Journal of Hematology & Thromboembolic Diseases
Open Access

ISSN: 2329-8790

+44 1478 350008

DNA-poor space in Hodgkin′s lymphoma nuclei


International Conference on Leukemia and Hematologic Oncology

October 17-18, 2016 Rome, Italy

Sabine Mai and Hans Knecht

University of Manitoba, Canada
McGill University, Canada

Scientific Tracks Abstracts: J Hematol Thrombo Dis

Abstract :

Our recent quantitative super-resolution imaging of Hodgkin��?s lymphoma (HL) nuclei demonstrated that there are significant differences in DNA-free/poor nuclear spaces between lymphocytes and mono-nucleated Hodgkin (H) cells as well as multi-nucleated Reed Sternberg (RS) cells. In addition, this difference is present between mono- and multinucleated RS cells: The latter documents the increase in DNA-poor space even more. Circular DNA-poor spaces were first thought to be nucleoli; however, since neither nucleolin nor Upstream Binding Factor (UBF), a transcription factor residing in nucleoli, stained these spaces, their function is currently elusive. Using a 12 nucleotide peptide nucleic acid probe, we identified telomeric fragments in circular DNA-poor spaces of H and RS. In addition, these spaces contain promyelocytic leukemia protein. The latter is usually associated with telomeres. As we have shown earlier, HL is a disease of telomere and shelterin dysfunction. Therefore, HL may use DNA-poor space to store telomeric fragments, i.e. extremely short telomeres, so-called T-stumps.

Biography :

Email: Sabine.Mai@umanitoba.ca

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