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Yong Joo Yoon and Jin Yoon
Chonbuk National University, South Korea
Posters & Accepted Abstracts: J Clin Cell Immunol
Introduction: Antimicrobial factors and mast cells and melanocytes are closely related to the protection of the skin from a pathogenic insult. They detected in the body skin containing glands. The goal of this investigation was to identify the presence of melanocytes and mast cells, referring to the distribution of antimicrobial factors in the gland of the external auditory canal skin. Material & Methods: Normal human external auditory canal skins were assessed for immunohistochemical evidence of mast cells and melanocyte and antimicrobial factors (hBD-1 and hBD-2). Immunoreactivity was detected using a standard avidin-biotin complex peroxidase method (Vectastain Universal Ellite ABC kit, Vector Laboratories). Results: Mast cells were mainly observed in the ceruminous gland cell areas. Dendritic melanocytes were found in the subepithelial space. The positive staining of hBD-1 was most intense in the ceruminous gland regions. Conclusion: These findings suggest that mast cells in the normal external auditory canal skin may somehow be involved in secretion of antimicrobial peptides in the ceruminous gland.