ISSN: 2471-9315
Nasrin Sultana, Jun Zhao, Yuanfeng Cai, Mohammad Faheem, Xianlong Peng and Zhongjun Jia
Institute of Soil Science-Chinese Academy of Sciences, China
University of Chinese Academy of Sciences, China
Northeast Agricultural University, China
Posters & Accepted Abstracts: Appli Micro
Methane is a potent greenhouse gas in the atmosphere causes global warming. Methanotrophic Bacteria (MOB) have the capacity to alleviate CH4 emissions from rice based ecosystems to the atmosphere. In this study, we provide molecular evidence for distinctly different phylotypes of methanotrophs through DNA-based Stable Isotope Probing (SIP) and high throughput MiSeq amplicon sequencing of the 16S rRNA and pmoA genes in paddy soils from cold-temperate region in China. To know the active MOB in total microbimes and their pmoA genotypes, we incubated four paddy soils with ambient CH4, high 12C-CH4 and 13C-CH4 amendment. The results showed strong activity of MOB and their ability of methane consumption in cold-temperate rice fields. The pmoA gene copies increased significantly with high 13C-CH4 incubated microcosms and reached the peak in heavy DNA fractions suggesting strong labeling of active MOB community. The pmoA and 16S rRNA gene sequences showed a high diversity of active methanotrophs and it seems that type-I was leading than type-II in three soils while only ones soil exhibited high abundance of type II- like methanotrophs. With all 13C-labeled methanotrophic 16S rRNA and pmoA gene sequences demonstrated that active MOB were affiliated with type-Ia including genera Methylobacter, Methylosarcina, Methylocaldum and Methylmicrobium while Methylocystis and Methylosinus-related to type-II methanotrophs. So, our study suggests that a wide variety of methanotrophs can modulate soil methane oxidation in cold-temperate rice fields that might be able to reducing the methane emissions to the atmosphere.
Nasrin Sultana completed Ph.D. at Institute of Soil Science-Chinese Academy of Sciences, China. Her research interest is on Environmental microbiology